Publications from 2019 

Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Preclinical Cancer Drug Cardiotoxicity Testing: A Scientific Statement From the American Heart Association.
Gintant G, Burridge P, Gepstein L, Harding S, Herron T, Hong C, Jalife J, Wu JC.
Circ Res. 2019 Oct 25;125(10):e75-e92. doi: 10.1161/RES.0000000000000291. Epub 2019 Sep 19. PMID: 31533542 Free PMC article. Review.

Characterization of acute TLR-7 agonist-induced hemorrhagic myocarditis in mice by multiparametric quantitative cardiac magnetic resonance imaging.
Baxan N, Papanikolaou A, Salles-Crawley I, Lota A, Chowdhury R, Dubois O, Branca J, Hasham MG, Rosenthal N, Prasad SK, Zhao L, Harding SE, Sattler S.
Dis Model Mech. 2019 Aug 16;12(8):dmm040725. doi: 10.1242/dmm.040725.
PMID: 31324689 Free PMC article.

Multi-cellularity in cardiac tissue engineering, how close are we to native heart tissue?
Owen TJ, Harding SE.
J Muscle Res Cell Motil. 2019 Jun;40(2):151-157. doi: 10.1007/s10974-019-09528-8. Epub 2019 Jun 20. PMID: 31222588 Free PMC article. Review.

Biomimetic electromechanical stimulation to maintain adult myocardial slices in vitro.
Watson SA, Duff J, Bardi I, Zabielska M, Atanur SS, Jabbour RJ, Simon A, Tomas A, Smolenski RT, Harding SE, Perbellini F, Terracciano CM.
Nat Commun. 2019 May 15;10(1):2168. doi: 10.1038/s41467-019-10175-3. PMID: 31092830 Free PMC article

Proteomic Analysis Reveals Temporal Changes in Protein Expression in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes In Vitro.
Hellen N, Pinto Ricardo C, Vauchez K, Whiting G, Wheeler JX, Harding SE.
Stem Cells Dev. 2019 May 1;28(9):565-578. doi: 10.1089/scd.2018.0210. Epub 2019 Mar 20.
PMID: 30755138

Allowance for diastolic interval distinguishes Na channel block in iPSC-derived cardiomyocytes.
Smith G, MacQuaide N, Nichols J.
J Pharmacol Toxicol Methods. 2019 Sep-Oct;99:106595. doi: 10.1016/j.vascn.2019.05.038. Epub 2019 Oct 17.
PMID: 31963064 No abstract available

Comparison of the effects of omecamtiv mercabil on excitation contraction coupling between isolated rabbit ventricular myocytes and human iPSC-derived cardiomyocytes.
McGlynn K, Firth K, MacQuaide N, Smith G.
J Pharmacol Toxicol Methods. 2019 Sep-Oct;99:106595. doi: 10.1016/j.vascn.2019.05.039. Epub 2019 Oct 17.
PMID: 31963062 No abstract available

Allowance for diastolic interval distinguishes Na channel block in iPSC-derived cardiomyocytes.
Smith G, MacQuaide N, Nichols J.
J Pharmacol Toxicol Methods. 2019 Sep-Oct;99:106595. doi: 10.1016/j.vascn.2019.05.172. Epub 2019 Oct 17. PMID: 31962929 No abstract available

GLUT4 expression and glucose transport in human induced pluripotent stem cell-derived cardiomyocytes.
Bowman PRT, Smith GL, Gould GW.
PLoS One. 2019 Jul 25;14(7):e0217885. doi: 10.1371/journal.pone.0217885. eCollection 2019.
PMID: 31344028 Free PMC article

Electrophysiological characterization of drug response in hSC-derived cardiomyocytes using voltage-sensitive optical platforms.
Pfeiffer-Kaushik ER, Smith GL, Cai B, Dempsey GT, Hortigon-Vinagre MP, Zamora V, Feng S, Ingermanson R, Zhu R, Hariharan V, Nguyen C, Pierson J, Gintant GA, Tung L.
J Pharmacol Toxicol Methods. 2019 Sep-Oct;99:106612. doi: 10.1016/j.vascn.2019.106612. Epub 2019 Jul 15. PMID: 31319140

Myocardial Slices: an Intermediate Complexity Platform for Translational Cardiovascular Research.
Watson SA, Terracciano CM, Perbellini F.
Cardiovasc Drugs Ther. 2019 Apr;33(2):239-244. doi: 10.1007/s10557-019-06853-5.
PMID: 30671746 Free PMC article. Review

MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In Vivo.
Fiedler LR, Chapman K, Xie M, Maifoshie E, Jenkins M, Golforoush PA, Bellahcene M, Noseda M, Faust D, Jarvis A, Newton G, Paiva MA, Harada M, Stuckey DJ, Song W, Habib J, Narasimhan P, Aqil R, Sanmugalingam D, Yan R, Pavanello L, Sano M, Wang SC, Sampson RD, Kanayaganam S, Taffet GE, Michael LH, Entman ML, Tan TH, Harding SE, Low CMR, Tralau-Stewart C, Perrior T, Schneider MD.
Cell Stem Cell. 2019 Apr 4;24(4):579-591.e12. doi: 10.1016/j.stem.2019.01.013. Epub 2019 Mar 7.
PMID: 30853557 Free PMC article.

A Software Tool for High-Throughput Real-Time Measurement of Intensity-Based Ratio-Metric FRET.
Ramuz M, Hasan A, Gruscheski L, Diakonov I, Pavlaki N, Nikolaev VO, Harding S, Dunsby C, Gorelik J.
Cells. 2019 Nov 29;8(12):1541. doi: 10.3390/cells8121541. PMID: 31795419 Free PMC article.

Phosphomimetic cardiac myosin-binding protein C partially rescues a cardiomyopathy phenotype in murine engineered heart tissue.
Dutsch A, Wijnker PJM, Schlossarek S, Friedrich FW, Krämer E, Braren I, Hirt MN, Brenière-Letuffe D, Rhoden A, Mannhardt I, Eschenhagen T, Carrier L, Mearini G.
Sci Rep. 2019 Dec 3;9(1):18152. doi: 10.1038/s41598-019-54665-2.
PMID: 31796859 Free PMC article.

A magnetics-based approach for fine-tuning afterload in engineered heart tissues.
Rodriguez ML, Werner TR, Becker B, Eschenhagen T, Hirt MN.
ACS Biomater Sci Eng. 2019 Jul 8;5(7):3663-3675. doi: 10.1021/acsbiomaterials.8b01568. Epub 2019 Jun 11. PMID: 31637285 Free PMC article.

Blockade of miR-140-3p prevents functional deterioration in afterload-enhanced engineered heart tissue.
Werner TR, Kunze AC, Stenzig J, Eschenhagen T, Hirt MN.
Sci Rep. 2019 Aug 7;9(1):11494. doi: 10.1038/s41598-019-46818-0.
PMID: 31391475 Free PMC article.

Author Correction: Differentiation of cardiomyocytes and generation of human engineered heart tissue.
Breckwoldt K, Brenière-Letuffe D, Mannhardt I, Schulze T, Ulmer B, Werner T, Benzin A, Klampe B, Reinsch MC, Laufer S, Shibamiya A, Prondzynski M, Mearini G, Schade D, Fuchs S, Neuber C, Krämer E, Saleem U, Schulze ML, Rodriguez ML, Eschenhagen T, Hansen A.
Nat Protoc. 2019 Sep;14(9):2748. doi: 10.1038/s41596-019-0228-5. PMID: 31384055

Human iPS cell-derived engineered heart tissue does not affect ventricular arrhythmias in a guinea pig cryo-injury model.
Pecha S, Yorgan K, Röhl M, Geertz B, Hansen A, Weinberger F, Sehner S, Ehmke H, Reichenspurner H, Eschenhagen T, Schwoerer AP.
Sci Rep. 2019 Jul 8;9(1):9831. doi: 10.1038/s41598-019-46409-z. PMID: 31285568 Free PMC article

Dissecting hiPSC-CM pacemaker function in a cardiac organoid model.
Schulze ML, Lemoine MD, Fischer AW, Scherschel K, David R, Riecken K, Hansen A, Eschenhagen T, Ulmer BM.
Biomaterials. 2019 Jun;206:133-145. doi: 10.1016/j.biomaterials.2019.03.023. Epub 2019 Mar 21.
PMID: 30933775

Implantation of hiPSC-derived Cardiac-muscle Patches after Myocardial Injury in a Guinea Pig Model.
Castro L, Geertz B, Reinsch M, Aksehirlioglu B, Hansen A, Eschenhagen T, Reichenspurner H, Weinberger F, Pecha S. J Vis Exp. 2019 Mar 18;(145). doi: 10.3791/58810. PMID: 30933073

Heart Repair With Myocytes.
Eschenhagen T, Weinberger F.
Circ Res. 2019 Mar 15;124(6):843-845. doi: 10.1161/CIRCRESAHA.118.314336.
PMID: 30870126 No abstract available

ESC Working Group on Cellular Biology of the Heart: position paper for Cardiovascular Research: tissue engineering strategies combined with cell therapies for cardiac repair in ischaemic heart disease and heart failure.
Madonna R, Van Laake LW, Botker HE, Davidson SM, De Caterina R, Engel FB, Eschenhagen T, Fernandez-Aviles F, Hausenloy DJ, Hulot JS, Lecour S, Leor J, Menasché P, Pesce M, Perrino C, Prunier F, Van Linthout S, Ytrehus K, Zimmermann WH, Ferdinandy P, Sluijter JPG.
Cardiovasc Res. 2019 Mar 1;115(3):488-500. doi: 10.1093/cvr/cvz010. PMID: 30657875 Free PMC article. Review.

Piezo-bending actuators for isometric or auxotonic contraction analysis of engineered heart tissue.
Mannhardt I, Warncke C, Trieu HK, Müller J, Eschenhagen T.
J Tissue Eng Regen Med. 2019 Jan;13(1):3-11. doi: 10.1002/term.2755. Epub 2018 Nov 26.
PMID: 30334614

Cardiomyopathy phenotypes in human-induced pluripotent stem cell-derived cardiomyocytes-a systematic review.
Eschenhagen T, Carrier L.
Pflugers Arch. 2019 May;471(5):755-768. doi: 10.1007/s00424-018-2214-0. Epub 2018 Oct 15.
PMID: 30324321 Free PMC article

High-Throughput Phenotyping Toolkit for Characterizing Cellular Models of Hypertrophic Cardiomyopathy In Vitro.
Mosqueira D, Lis-Slimak K, Denning C.
Methods Protoc. 2019 Oct 26;2(4):83. doi: 10.3390/mps2040083. PMID: 31717790 Free PMC article

Variable expression and silencing of CRISPR-Cas9 targeted transgenes identifies the AAVS1 locus as not an entirely safe harbour.
Bhagwan JR, Collins E, Mosqueira D, Bakar M, Johnson BB, Thompson A, Smith JGW, Denning C.
F1000Res. 2019 Nov 12;8:1911. doi: 10.12688/f1000research.19894.2. eCollection 2019.
PMID: 32789000 Free PMC article

Simultaneous measurement of contraction, voltage and calcium in HIPSC-CMS for the detection of inotropic effects under blinded conditions.
de Korte T, van Meer B, Garcia AK, Tertoolen L, Clements PJ, Bahinski A, Rossman EI, Xu X, Turner S, Denning C, Vlaming M, Braam S, Mummery C.
J Pharmacol Toxicol Methods. 2019 Sep-Oct;99:106595. doi: 10.1016/j.vascn.2019.05.170. Epub 2019 Oct 17. PMID: 31962931 No abstract available

Polymer Microparticles with Defined Surface Chemistry and Topography Mediate the Formation of Stem Cell Aggregates and Cardiomyocyte Function.
Alvarez-Paino M, Amer MH, Nasir A, Cuzzucoli Crucitti V, Thorpe J, Burroughs L, Needham D, Denning C, Alexander MR, Alexander C, Rose FRAJ.
ACS Appl Mater Interfaces. 2019 Sep 25;11(38):34560-34574. doi: 10.1021/acsami.9b04769. Epub 2019 Sep 10.
PMID: 31502820

Modeling Hypertrophic Cardiomyopathy: Mechanistic Insights and Pharmacological Intervention.
Mosqueira D, Smith JGW, Bhagwan JR, Denning C.
Trends Mol Med. 2019 Sep;25(9):775-790. doi: 10.1016/j.molmed.2019.06.005. Epub 2019 Jul 17.
PMID: 31324451 Review

Investigating the Complex Arrhythmic Phenotype Caused by the Gain-of-Function Mutation KCNQ1-G229D.
Zhou X, Bueno-Orovio A, Schilling RJ, Kirkby C, Denning C, Rajamohan D, Burrage K, Tinker A, Rodriguez B, Harmer SC.
Front Physiol. 2019 Mar 18;10:259. doi: 10.3389/fphys.2019.00259. eCollection 2019.
PMID: 30967788 Free PMC article

Surface plasmon resonance imaging of excitable cells.
Howe CL, Webb KF, Abayzeed SA, Anderson DJ, Denning C, Russell NA.
J Phys D Appl Phys. 2019 Mar 6;52(10):104001. doi: 10.1088/1361-6463/aaf849. Epub 2019 Jan 4.
PMID: 30867618 Free PMC article

Void-free 3D Bioprinting for In-situ Endothelialization and Microfluidic Perfusion.
Ouyang L, Armstrong JPK, Chen Q, Lin Y, Stevens MM.
Adv Funct Mater. 2019 Nov 11;30(1):1908349. doi: 10.1002/adfm.201908349. eCollection 2020 Jan 3.
PMID: 33071714 Free PMC article

The Design and In Vivo Testing of a Locally Stiffness-Matched Porous Scaffold.
Ghouse S, Reznikov N, Boughton OR, Babu S, Geoffrey Ng KC, Blunn G, Cobb JP, Stevens MM, Jeffers JRT.
Appl Mater Today. 2019 May 30;15:377-388. doi: 10.1016/j.apmt.2019.02.017. Epub 2019 Mar 14.
PMID: 31281871 Free PMC article.

Toward Regeneration of the Heart: Bioengineering Strategies for Immunomodulation.
Ferrini A, Stevens MM, Sattler S, Rosenthal N.
Front Cardiovasc Med. 2019 Mar 21;6:26. doi: 10.3389/fcvm.2019.00026. eCollection 2019.
PMID: 30949485 Free PMC article. Review

Buoyancy-Driven Gradients for Biomaterial Fabrication and Tissue Engineering.
Li C, Ouyang L, Pence IJ, Moore AC, Lin Y, Winter CW, Armstrong JPK, Stevens MM.
Adv Mater. 2019 Apr;31(17):e1900291. doi: 10.1002/adma.201900291. Epub 2019 Mar 7.
PMID: 30844123 Free PMC article

Emerging Technologies for Tissue Engineering: From Gene Editing to Personalized Medicine.
Armstrong JPK, Stevens MM.
Tissue Eng Part A. 2019 May;25(9-10):688-692. doi: 10.1089/ten.TEA.2019.0026.
PMID: 30794069 Free PMC article. Review

Publications from 2018

Isogenic Pairs of hiPSC-CMs with Hypertrophic Cardiomyopathy/LVNC-Associated ACTC1 E99K Mutation Unveil Differential Functional Deficits.
Smith JGW, Owen T, Bhagwan JR, Mosqueira D, Scott E, Mannhardt I, Patel A, Barriales-Villa R, Monserrat L, Hansen A, Eschenhagen T, Harding SE, Marston S, Denning C.
Stem Cell Reports. 2018 Nov 13;11(5):1226-1243. doi: 10.1016/j.stemcr.2018.10.006. Epub 2018 Nov 1. PMID: 30392975 Free PMC article.

Immunomodulatory interventions in myocardial infarction and heart failure: a systematic review of clinical trials and meta-analysis of IL-1 inhibition.
Panahi M, Papanikolaou A, Torabi A, Zhang JG, Khan H, Vazir A, Hasham MG, Cleland JGF, Rosenthal NA, Harding SE, Sattler S.
Cardiovasc Res. 2018 Sep 1;114(11):1445-1461. doi: 10.1093/cvr/cvy145.
PMID: 30010800 Free PMC article.

Investigation of cardiac fibroblasts using myocardial slices.
Perbellini F, Watson SA, Scigliano M, Alayoubi S, Tkach S, Bardi I, Quaife N, Kane C, Dufton NP, Simon A, Sikkel MB, Faggian G, Randi AM, Gorelik J, Harding SE, Terracciano CM.
Cardiovasc Res. 2018 Jan 1;114(1):77-89. doi: 10.1093/cvr/cvx152.
PMID: 29016704 Free PMC article.

Poly(3-hydroxyoctanoate), a promising new material for cardiac tissue engineering.
Bagdadi AV, Safari M, Dubey P, Basnett P, Sofokleous P, Humphrey E, Locke I, Edirisinghe M, Terracciano C, Boccaccini AR, Knowles JC, Harding SE, Roy I.
J Tissue Eng Regen Med. 2018 Jan;12(1):e495-e512. doi: 10.1002/term.2318. Epub 2017 Aug 23.
PMID: 27689781

International Multisite Study of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Drug Proarrhythmic Potential Assessment.
Blinova K, Dang Q, Millard D, Smith G, Pierson J, Guo L, Brock M, Lu HR, Kraushaar U, Zeng H, Shi H, Zhang X, Sawada K, Osada T, Kanda Y, Sekino Y, Pang L, Feaster TK, Kettenhofen R, Stockbridge N, Strauss DG, Gintant G.
Cell Rep. 2018 Sep 25;24(13):3582-3592. doi: 10.1016/j.celrep.2018.08.079.
PMID: 30257217 Free PMC article.

Quantification of Muscle Contraction In Vitro and In Vivo Using MUSCLEMOTION Software: From Stem Cell-Derived Cardiomyocytes to Zebrafish and Human Hearts.
van Meer BJ, Sala L, Tertoolen LGJ, Smith GL, Burton FL, Mummery CL.
Curr Protoc Hum Genet. 2018 Oct;99(1):e67. doi: 10.1002/cphg.67. Epub 2018 Sep 25. PMID: 30253059

MUSCLEMOTION: A Versatile Open Software Tool to Quantify Cardiomyocyte and Cardiac Muscle Contraction In Vitro and In Vivo.
Sala L, van Meer BJ, Tertoolen LGJ, Bakkers J, Bellin M, Davis RP, Denning C, Dieben MAE, Eschenhagen T, Giacomelli E, Grandela C, Hansen A, Holman ER, Jongbloed MRM, Kamel SM, Koopman CD, Lachaud Q, Mannhardt I, Mol MPH, Mosqueira D, Orlova VV, Passier R, Ribeiro MC, Saleem U, Smith GL, Burton FL, Mummery CL.
Circ Res. 2018 Feb 2;122(3):e5-e16. doi: 10.1161/CIRCRESAHA.117.312067. Epub 2017 Dec 27.
PMID: 29282212 Free PMC article. Review

Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning.
Armstrong JPK, Puetzer JL, Serio A, Guex AG, Kapnisi M, Breant A, Zong Y, Assal V, Skaalure SC, King O, Murty T, Meinert C, Franklin AC, Bassindale PG, Nichols MK, Terracciano CM, Hutmacher DW, Drinkwater BW, Klein TJ, Perriman AW, Stevens MM.
Adv Mater. 2018 Oct;30(43):e1802649. doi: 10.1002/adma.201802649. Epub 2018 Sep 12.
PMID: 30277617 Free PMC article

Human Cardiac Fibroblasts Engage the Sarcoplasmic Reticulum in Induced Pluripotent Stem Cell-Derived Cardiomyocyte Excitation-Contraction Coupling.
Kane C, Terracciano CM.
J Am Coll Cardiol. 2018 Aug 28;72(9):1061-1063. doi: 10.1016/j.jacc.2018.06.028.
PMID: 30139435 No abstract available

Concurrent micro- to macro-cardiac electrophysiology in myocyte cultures and human heart slices.
Chowdhury RA, Tzortzis KN, Dupont E, Selvadurai S, Perbellini F, Cantwell CD, Ng FS, Simon AR, Terracciano CM, Peters NS.
Sci Rep. 2018 May 2;8(1):6947. doi: 10.1038/s41598-018-25170-9. PMID: 29720607 Free PMC article

A flexible polyaniline-based bioelectronic patch.
Cui C , Faraji N , Lauto A , Travaglini L , Tonkin J , Mahns D , Humphrey E , Terracciano C , Gooding JJ , Seidel J , Mawad D .
Biomater Sci. 2018 Feb 27;6(3):493-500. doi: 10.1039/c7bm00880e. PMID: 29363686

Functionally Conserved Noncoding Regulators of Cardiomyocyte Proliferation and Regeneration in Mouse and Human.
Adamowicz M, Morgan CC, Haubner BJ, Noseda M, Collins MJ, Abreu Paiva M, Srivastava PK, Gellert P, Razzaghi B, O'Gara P, Raina P, Game L, Bottolo L, Schneider MD, Harding SE, Penninger J, Aitman TJ.
Circ Genom Precis Med. 2018 Feb;11(2):e001805. doi: 10.1161/CIRCGEN.117.001805. Epub 2018 Feb 1.
PMID: 32966096

Correction: Analysis of Tyrosine Kinase Inhibitor-Mediated Decline in Contractile Force in Rat Engineered Heart Tissue.
Jacob F, Yonis AY, Cuello F, Luther P, Schulze T, Eder A, Streichert T, Mannhardt I, Hirt MN, Schaaf S, Stenzig J, Force T, Eschenhagen T, Hansen A.
PLoS One. 2018 Nov 27;13(11):e0208342. doi: 10.1371/journal.pone.0208342. eCollection 2018.
PMID: 30481219 Free PMC article

Atrial-like Engineered Heart Tissue: An In Vitro Model of the Human Atrium.
Lemme M, Ulmer BM, Lemoine MD, Zech ATL, Flenner F, Ravens U, Reichenspurner H, Rol-Garcia M, Smith G, Hansen A, Christ T, Eschenhagen T.
Stem Cell Reports. 2018 Dec 11;11(6):1378-1390. doi: 10.1016/j.stemcr.2018.10.008. Epub 2018 Nov 8.
PMID: 30416051 Free PMC article

Normalization of force to muscle cross-sectional area: A helpful attempt to reduce data scattering in contractility studies?
Pecha S, Koivumäki J, Geelhoed B, Kempe R, Berk E, Engel A, Reichenspurner H, Eschenhagen T, Ravens U, Kaumann A, Christ T.
Acta Physiol (Oxf). 2018 Dec;224(4):e13202. doi: 10.1111/apha.13202.
PMID: 30338659 No abstract available

Rat atrial engineered heart tissue: a new in vitro model to study atrial biology.
Krause J, Löser A, Lemoine MD, Christ T, Scherschel K, Meyer C, Blankenberg S, Zeller T, Eschenhagen T, Stenzig J.
Basic Res Cardiol. 2018 Sep 3;113(5):41. doi: 10.1007/s00395-018-0701-2.
PMID: 30178427

Exaggerated Cardiotoxicity of Sunitinib in Stressed 3-Dimensional Heart Muscles.
Eschenhagen T.
JACC Basic Transl Sci. 2018 May 30;3(2):277-279. doi: 10.1016/j.jacbts.2018.01.011. eCollection 2018 Apr.
PMID: 30062213 Free PMC article

Human Induced Pluripotent Stem Cell-Derived Engineered Heart Tissue as a Sensitive Test System for QT Prolongation and Arrhythmic Triggers.
Lemoine MD, Krause T, Koivumäki JT, Prondzynski M, Schulze ML, Girdauskas E, Willems S, Hansen A, Eschenhagen T, Christ T.
Circ Arrhythm Electrophysiol. 2018 Jul;11(7):e006035. doi: 10.1161/CIRCEP.117.006035.
PMID: 29925535

Clonal dynamics studied in cultured induced pluripotent stem cells reveal major growth imbalances within a few weeks.
Brenière-Letuffe D, Domke-Shibamiya A, Hansen A, Eschenhagen T, Fehse B, Riecken K, Stenzig J.
Stem Cell Res Ther. 2018 Jun 18;9(1):165. doi: 10.1186/s13287-018-0893-2.
PMID: 29914569 Free PMC article

CRISPR/Cas9 editing in human pluripotent stem cell-cardiomyocytes highlights arrhythmias, hypocontractility, and energy depletion as potential therapeutic targets for hypertrophic cardiomyopathy.
Mosqueira D, Mannhardt I, Bhagwan JR, Lis-Slimak K, Katili P, Scott E, Hassan M, Prondzynski M, Harmer SC, Tinker A, Smith JGW, Carrier L, Williams PM, Gaffney D, Eschenhagen T, Hansen A, Denning C.
Eur Heart J. 2018 Nov 14;39(43):3879-3892. doi: 10.1093/eurheartj/ehy249.
PMID: 29741611 Free PMC article

Three-Dimensional Human iPSC-Derived Artificial Skeletal Muscles Model Muscular Dystrophies and Enable Multilineage Tissue Engineering.
Maffioletti SM, Sarcar S, Henderson ABH, Mannhardt I, Pinton L, Moyle LA, Steele-Stallard H, Cappellari O, Wells KE, Ferrari G, Mitchell JS, Tyzack GE, Kotiadis VN, Khedr M, Ragazzi M, Wang W, Duchen MR, Patani R, Zammit PS, Wells DJ, Eschenhagen T, Tedesco FS.
Cell Rep. 2018 Apr 17;23(3):899-908. doi: 10.1016/j.celrep.2018.03.091.
PMID: 29669293 Free PMC article

Contractile Work Contributes to Maturation of Energy Metabolism in hiPSC-Derived Cardiomyocytes.
Ulmer BM, Stoehr A, Schulze ML, Patel S, Gucek M, Mannhardt I, Funcke S, Murphy E, Eschenhagen T, Hansen A.
Stem Cell Reports. 2018 Mar 13;10(3):834-847. doi: 10.1016/j.stemcr.2018.01.039. Epub 2018 Mar 1.
PMID: 29503093 Free PMC article

Low Resting Membrane Potential and Low Inward Rectifier Potassium Currents Are Not Inherent Features of hiPSC-Derived Cardiomyocytes.
Horváth A, Lemoine MD, Löser A, Mannhardt I, Flenner F, Uzun AU, Neuber C, Breckwoldt K, Hansen A, Girdauskas E, Reichenspurner H, Willems S, Jost N, Wettwer E, Eschenhagen T, Christ T.
Stem Cell Reports. 2018 Mar 13;10(3):822-833. doi: 10.1016/j.stemcr.2018.01.012. Epub 2018 Feb 8.
PMID: 29429959 Free PMC article

Exosomes: Basic Biology and Technological Advancements Suggesting Their Potential as Ischemic Heart Disease Therapeutics.
Shanmuganathan M, Vughs J, Noseda M, Emanueli C.
Front Physiol. 2018 Nov 19;9:1159. doi: 10.3389/fphys.2018.01159. eCollection 2018.
PMID: 30524292 Free PMC article. Review

Auxetic Cardiac Patches with Tunable Mechanical and Conductive Properties toward Treating Myocardial Infarction.
Kapnisi M, Mansfield C, Marijon C, Guex AG, Perbellini F, Bardi I, Humphrey EJ, Puetzer JL, Mawad D, Koutsogeorgis DC, Stuckey DJ, Terracciano CM, Harding SE, Stevens MM.
Adv Funct Mater. 2018 May 24;28(21):1800618. doi: 10.1002/adfm.201800618.
PMID: 29875619 Free PMC article

Development and validation of broad-spectrum magnetic particle labelling processes for cell therapy manufacturing.
Harrison R, Lugo Leija HA, Strohbuecker S, Crutchley J, Marsh S, Denning C, El Haj A, Sottile V.
Stem Cell Res Ther. 2018 Sep 26;9(1):248. doi: 10.1186/s13287-018-0968-0.
PMID: 30257709 Free PMC article

Simplified Footprint-Free Cas9/CRISPR Editing of Cardiac-Associated Genes in Human Pluripotent Stem Cells.
Kondrashov A, Duc Hoang M, Smith JGW, Bhagwan JR, Duncan G, Mosqueira D, Munoz MB, Vo NTN, Denning C.
Stem Cells Dev. 2018 Mar 15;27(6):391-404. doi: 10.1089/scd.2017.0268. Epub 2018 Mar 12.
PMID: 29402189 Free PMC article

Single Particle Automated Raman Trapping Analysis.
Penders J, Pence IJ, Horgan CC, Bergholt MS, Wood CS, Najer A, Kauscher U, Nagelkerke A, Stevens MM.
Nat Commun. 2018 Oct 15;9(1):4256. doi: 10.1038/s41467-018-06397-6.
PMID: 30323298 Free PMC article

Elastic serum-albumin based hydrogels: mechanism of formation and application in cardiac tissue engineering.
Amdursky N, Mazo MM, Thomas MR, Humphrey EJ, Puetzer JL, St-Pierre JP, Skaalure SC, Richardson RM, Terracciano CM, Stevens MM.
J Mater Chem B. 2018 Sep 21;6(35):5604-5612. doi: 10.1039/C8TB01014E. Epub 2018 Aug 23.
PMID: 30283632 Free PMC article

Engineering Extracellular Vesicles with the Tools of Enzyme Prodrug Therapy.
Fuhrmann G, Chandrawati R, Parmar PA, Keane TJ, Maynard SA, Bertazzo S, Stevens MM.
Adv Mater. 2018 Apr;30(15):e1706616. doi: 10.1002/adma.201706616. Epub 2018 Feb 23.
PMID: 29473230 Free PMC article

Cell-geometry-dependent changes in plasma membrane order direct stem cell signalling and fate.
von Erlach TC, Bertazzo S, Wozniak MA, Horejs CM, Maynard SA, Attwood S, Robinson BK, Autefage H, Kallepitis C, Del Río Hernández A, Chen CS, Goldoni S, Stevens MM.
Nat Mater. 2018 Mar;17(3):237-242. doi: 10.1038/s41563-017-0014-0. Epub 2018 Feb 12.
PMID: 29434303 Free PMC article.

Scarring vs. functional healing: Matrix-based strategies to regulate tissue repair.
Keane TJ, Horejs CM, Stevens MM.
Adv Drug Deliv Rev. 2018 Apr;129:407-419. doi: 10.1016/j.addr.2018.02.002. Epub 2018 Feb 6.
PMID: 29425770 Free PMC article. Review.

Publications from 2017

The adaptive immune response to cardiac injury-the true roadblock to effective regenerative therapies?
Sattler S, Fairchild P, Watt FM, Rosenthal N, Harding SE.
NPJ Regen Med. 2017 Jun 19;2:19. doi: 10.1038/s41536-017-0022-3. eCollection 2017.
PMID: 29302355 Free PMC article. Review

Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Encapsulating Bioactive Hydrogels Improve Rat Heart Function Post Myocardial Infarction.
Chow A, Stuckey DJ, Kidher E, Rocco M, Jabbour RJ, Mansfield CA, Darzi A, Harding SE, Stevens MM, Athanasiou T.
Stem Cell Reports. 2017 Nov 14;9(5):1415-1422. doi: 10.1016/j.stemcr.2017.09.003. Epub 2017 Oct 5. PMID: 28988988 Free PMC article

Overexpression of hypoxia-inducible factor 1 alpha improves immunomodulation by dental mesenchymal stem cells.
Martinez VG, Ontoria-Oviedo I, Ricardo CP, Harding SE, Sacedon R, Varas A, Zapata A, Sepulveda P, Vicente A.
Stem Cell Res Ther. 2017 Sep 29;8(1):208. doi: 10.1186/s13287-017-0659-2. PMID: 28962641 Free PMC article

Induced pluripotent stem cell modelling of HLHS underlines the contribution of dysfunctional NOTCH signalling to impaired cardiogenesis.
Yang C, Xu Y, Yu M, Lee D, Alharti S, Hellen N, Ahmad Shaik N, Banaganapalli B, Sheikh Ali Mohamoud H, Elango R, Przyborski S, Tenin G, Williams S, O'Sullivan J, Al-Radi OO, Atta J, Harding SE, Keavney B, Lako M, Armstrong L.
Hum Mol Genet. 2017 Aug 15;26(16):3031-3045. doi: 10.1093/hmg/ddx140.
PMID: 28521042 Free PMC article

Systemic autoimmunity induced by the TLR7/8 agonist Resiquimod causes myocarditis and dilated cardiomyopathy in a new mouse model of autoimmune heart disease.
Hasham MG, Baxan N, Stuckey DJ, Branca J, Perkins B, Dent O, Duffy T, Hameed TS, Stella SE, Bellahcene M, Schneider MD, Harding SE, Rosenthal N, Sattler S.
Dis Model Mech. 2017 Mar 1;10(3):259-270. doi: 10.1242/dmm.027409.
PMID: 28250051 Free PMC article

Tailoring Mathematical Models to Stem-Cell Derived Cardiomyocyte Lines Can Improve Predictions of Drug-Induced Changes to Their Electrophysiology.
Lei CL, Wang K, Clerx M, Johnstone RH, Hortigon-Vinagre MP, Zamora V, Allan A, Smith GL, Gavaghan DJ, Mirams GR, Polonchuk L.
Front Physiol. 2017 Dec 12;8:986. doi: 10.3389/fphys.2017.00986. eCollection 2017.
PMID: 29311950 Free PMC article

Drug-Mediated Shortening of Action Potentials in LQTS2 Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Duncan G, Firth K, George V, Hoang MD, Staniforth A, Smith G, Denning C.
Stem Cells Dev. 2017 Dec 1;26(23):1695-1705. doi: 10.1089/scd.2017.0172. Epub 2017 Oct 9.
PMID: 28992755 Free PMC article

Preparation of viable adult ventricular myocardial slices from large and small mammals.
Watson SA, Scigliano M, Bardi I, Ascione R, Terracciano CM, Perbellini F.
Nat Protoc. 2017 Dec;12(12):2623-2639. doi: 10.1038/nprot.2017.139. Epub 2017 Nov 30.
PMID: 29189769

Adult cardiac stem cells are multipotent and robustly myogenic: c-kit expression is necessary but not sufficient for their identification.
Vicinanza C, Aquila I, Scalise M, Cristiano F, Marino F, Cianflone E, Mancuso T, Marotta P, Sacco W, Lewis FC, Couch L, Shone V, Gritti G, Torella A, Smith AJ, Terracciano CM, Britti D, Veltri P, Indolfi C, Nadal-Ginard B, Ellison-Hughes GM, Torella D.
Cell Death Differ. 2017 Dec;24(12):2101-2116. doi: 10.1038/cdd.2017.130. Epub 2017 Aug 11.
PMID: 28800128 Free PMC article

Free-of-Acrylamide SDS-based Tissue Clearing (FASTClear) for three dimensional visualization of myocardial tissue.
Perbellini F, Liu AKL, Watson SA, Bardi I, Rothery SM, Terracciano CM.
Sci Rep. 2017 Jul 12;7(1):5188. doi: 10.1038/s41598-017-05406-w.
PMID: 28701763 Free PMC article

Concise Review: Criteria for Chamber-Specific Categorization of Human Cardiac Myocytes Derived from Pluripotent Stem Cells.
Kane C, Terracciano CMN.
Stem Cells. 2017 Aug;35(8):1881-1897. doi: 10.1002/stem.2649. Epub 2017 Jun 27.
PMID: 28577296 Free PMC article. Review

Upstairs, Downstairs: Atrial and Ventricular Cardiac Myocytes from Human Pluripotent Stem Cells.
Schneider MD.
Cell Stem Cell. 2017 Aug 3;21(2):151-152. doi: 10.1016/j.stem.2017.07.006.
PMID: 28777937

Multimodal Hydrogel-Based Platform To Deliver and Monitor Cardiac Progenitor/Stem Cell Engraftment.
Speidel AT, Stuckey DJ, Chow LW, Jackson LH, Noseda M, Abreu Paiva M, Schneider MD, Stevens MM.
ACS Cent Sci. 2017 Apr 26;3(4):338-348. doi: 10.1021/acscentsci.7b00039. Epub 2017 Mar 30.
PMID: 28470052 Free PMC article.

Human iPSC-derived cardiomyocytes cultured in 3D engineered heart tissue show physiological upstroke velocity and sodium current density.
Lemoine MD, Mannhardt I, Breckwoldt K, Prondzynski M, Flenner F, Ulmer B, Hirt MN, Neuber C, Horváth A, Kloth B, Reichenspurner H, Willems S, Hansen A, Eschenhagen T, Christ T.
Sci Rep. 2017 Jul 14;7(1):5464. doi: 10.1038/s41598-017-05600-w.
PMID: 28710467 Free PMC article.

Cardiomyocyte Regeneration: A Consensus Statement.
Eschenhagen T, Bolli R, Braun T, Field LJ, Fleischmann BK, Frisén J, Giacca M, Hare JM, Houser S, Lee RT, Marbán E, Martin JF, Molkentin JD, Murry CE, Riley PR, Ruiz-Lozano P, Sadek HA, Sussman MA, Hill JA.
Circulation. 2017 Aug 15;136(7):680-686. doi: 10.1161/CIRCULATIONAHA.117.029343. Epub 2017 Jul 6.
PMID: 28684531 Free PMC article. Review. No abstract available.

Evaluation of MYBPC3 trans-Splicing and Gene Replacement as Therapeutic Options in Human iPSC-Derived Cardiomyocytes.
Prondzynski M, Krämer E, Laufer SD, Shibamiya A, Pless O, Flenner F, Müller OJ, Münch J, Redwood C, Hansen A, Patten M, Eschenhagen T, Mearini G, Carrier L.
Mol Ther Nucleic Acids. 2017 Jun 16;7:475-486. doi: 10.1016/j.omtn.2017.05.008. Epub 2017 May 17.
PMID: 28624223 Free PMC article

Differentiation of cardiomyocytes and generation of human engineered heart tissue.
Breckwoldt K, Letuffe-Brenière D, Mannhardt I, Schulze T, Ulmer B, Werner T, Benzin A, Klampe B, Reinsch MC, Laufer S, Shibamiya A, Prondzynski M, Mearini G, Schade D, Fuchs S, Neuber C, Krämer E, Saleem U, Schulze ML, Rodriguez ML, Eschenhagen T, Hansen A.
Nat Protoc. 2017 Jun;12(6):1177-1197. doi: 10.1038/nprot.2017.033. Epub 2017 May 11.
PMID: 28492526

Blinded Contractility Analysis in hiPSC-Cardiomyocytes in Engineered Heart Tissue Format: Comparison With Human Atrial Trabeculae.
Mannhardt I, Eder A, Dumotier B, Prondzynski M, Krämer E, Traebert M, Söhren KD, Flenner F, Stathopoulou K, Lemoine MD, Carrier L, Christ T, Eschenhagen T, Hansen A.
Toxicol Sci. 2017 Jul 1;158(1):164-175. doi: 10.1093/toxsci/kfx081.
PMID: 28453742 Free PMC article

Engineering Cardiac Muscle Tissue: A Maturating Field of Research.
Weinberger F, Mannhardt I, Eschenhagen T.
Circ Res. 2017 Apr 28;120(9):1487-1500. doi: 10.1161/CIRCRESAHA.117.310738.
PMID: 28450366 Review

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening.
Mannhardt I, Saleem U, Benzin A, Schulze T, Klampe B, Eschenhagen T, Hansen A.
J Vis Exp. 2017 Apr 15;(122):55461. doi: 10.3791/55461.
PMID: 28448053 Free PMC article.

Thymosin β4 Improves Differentiation and Vascularization of EHTs.
Ziegler T, Hinkel R, Stöhr A, Eschenhagen T, Laugwitz KL, le Noble F, David R, Hansen A, Kupatt C.
Stem Cells Int. 2017;2017:6848271. doi: 10.1155/2017/6848271. Epub 2017 Jan 16.
PMID: 28191018 Free PMC article.

Tumor Resection Recruits Effector T Cells and Boosts Therapeutic Efficacy of Encapsulated Stem Cells Expressing IFNβ in Glioblastomas.
Choi SH, Stuckey DW, Pignatta S, Reinshagen C, Khalsa JK, Roozendaal N, Martinez-Quintanilla J, Tamura K, Keles E, Shah K.
Clin Cancer Res. 2017 Nov 15;23(22):7047-7058. doi: 10.1158/1078-0432.CCR-17-0077. Epub 2017 Sep 14.
PMID: 28912136 Free PMC article.

Stem cell-derived models to improve mechanistic understanding and prediction of human drug-induced liver injury.
Goldring C, Antoine DJ, Bonner F, Crozier J, Denning C, Fontana RJ, Hanley NA, Hay DC, Ingelman-Sundberg M, Juhila S, Kitteringham N, Silva-Lima B, Norris A, Pridgeon C, Ross JA, Young RS, Tagle D, Tornesi B, van de Water B, Weaver RJ, Zhang F, Park BK.
Hepatology. 2017 Feb;65(2):710-721. doi: 10.1002/hep.28886. Epub 2016 Nov 30.
PMID: 27775817 Free PMC article. Review

---------------------------------------------------------------------------------