LSR ll

LSR II

405nM Violet laser

Collection filterMirrorFluorochromes
450/50 - Alexa Fluor 405, BD Horizon V450, BV 421, Calcein Violet, Calcein Violet 450AM, Celltrace Violet, CFP, DAPI, DyLight 405, DyeCycle Violet, eFluor 450, Fixable Violet Dead Cell Stain, Fixable Viability eFluor 450, FxCycle Violet, Hoechst (blue), Pacific Blue, PO-PRO-1, SYTOX Blue, VioBlue
525/50 475LP AmCyan, BD Horizon V500, Brilliant Violet 510, Fixable Aqua Dead cell stain, Fixable Viability eFluor 506, Krome Orange, Pacific Orange, Qdot 525, VioGreen
560/20* 550LP Qdot 565, Pacific Orange, Krome Orange 
585/15* 575LP Brilliant Violet 570, Fixable Yellow Dead Cell Stain, Qdot 585, Qdot 605
605/12* 595LP Brilliant Violet 605, eFluor605NC, Fixable Yellow Dead Cell Stain, Pacific Orange, Qdot 605
655/8* 635LP Brilliant Violet 650, Qdot 655
* These filters can be interchangeable- On the Violet laser BV 711 and Qdot 705 can be used with a 710/50 filter and a 685nm LP dichroic mirror. BV 785 and Qdot 800 can be used with a 780/60 filter and a 750nm LP dichroic mirror.
 
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
Summary of the table's contents

488nM Blue laser

Collection filterMirrorFluorochromes
530/30 505LP AlexaFluor 488, Calcein, CellVue Jade, CFSE, Cy2, DyLight 488, DyeCycle Green, eGFP,  eYFP , FITC, Fixable green dead cell stain, Fluo-3/Fluo-4, mCitrine, Oyster 488, Rhodamine 110,  Rhodamine 123,  SYTOX Green, TO-PRO-1, YO-PRO-1
575/26 550LP Cy3, DyeCycle Orange, Fura Red, Oyster550/555, PE, pHrodo, PI, SNARF (Low pH), SYTOX-Orange
610/20 600LP 7AAD, PE-Texas Red, PI, PE-CF594
660/20 650LP PE-Cy5, PI
695/40 685LP 7-AAD, DRAQ5, Dye Cycle Ruby, PerCP-Cy5.5, PerCP-eFluor710, PI 
780/60 755LP  PE-Alexa Fluor 750, PE-Cy7
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
Summary of the table's contents

 

 

633nM Red laser

Collection filterMirrorFluorochromes
660/20 - APC, Alexa Fluor 647, Cell Proliferation Dye eFluor 670, CellVue Maroon, CellVue Plum, Cy5, Cy5.5, DRAQ5, DRAQ7, Dylight 649, Dye Cycle Ruby, Fixable far red dead stain, Fixable Viability eFluor 660, Fx Cycle Far Red Stain, Oyster 650, SYTOX Red, TO-PRO-3, YO-PRO-3
730/45 710LP Alexa Fluor 700, APC-Cy5.5, CellVue Burgundy, DRAQ7, Dye Cycle Ruby
780/60 755LP Alexa Fluor 750, APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, APC-H7, APC-Vio770, CellVue NIR780, DRAQ7, Fixable near-IR dead stain,, Fixable Viability eFluor 780
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
Summary of the table's contents