Fortessa B
405nM Violet laser
| Collection filter | Mirror | Fluorochromes |
|---|---|---|
| 450/50 | - | Alexa Fluor 405, BD Horizon V450, BV 421, Calcein Violet, Calcein Violet 450AM, Celltrace Violet, CFP, DAPI, DyLight 405, DyeCycle Violet, eFluor 450, Fixable Violet Dead Cell Stain, Fixable Viability eFluor 450, FxCycle Violet, Hoechst (blue), Pacific Blue, PO-PRO-1, SYTOX Blue, VioBlue |
| 525/50 | 475LP | AmCyan, BD Horizon V500, Brilliant Violet 510, Fixable Aqua Dead cell stain, Fixable Viability eFluor 506, Krome Orange, Pacific Orange, Qdot 525, VioGreen |
| 605/12 | 595LP | Brilliant Violet 605, eFluor605NC, Fixable Yellow Dead Cell Stain, Pacific Orange, Qdot 605 |
| 655/8 | 635LP | Brilliant Violet 650, Qdot 655 |
| 710/50 | 690LP | Brilliant Violet 711 |
| 780/60 | 750LP | Brilliant Violet 786, Super Bright 780 |
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Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
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488nM Blue laser
| Collection filter | Mirror | Fluorochromes |
|---|---|---|
| 530/30 | 505LP | AlexaFluor 488, Calcein, CellVue Jade, CFSE, Cy2, DyLight 488, DyeCycle Green, eGFP, eYFP , FITC, Fixable green dead cell stain, Fluo-3/Fluo-4, mCitrine, Oyster 488, Rhodamine 110, Rhodamine 123, SYTOX Green, TO-PRO-1, YO-PRO-1 |
| 670/14 | 660LP | 7-AAD, PerCP, PI, (PE-Cy5**) |
| 710/40 | 685LP | 7-AAD, DRAQ5, Dye Cycle Ruby, PerCP-Cy5.5, PerCP-eFluor710, PI |
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**PE-Cy5 is not recommended for use with APC. Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
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561nM Yellow Green laser
| Collection filter | Mirror | Fluorochromes |
|---|---|---|
| 582/15 | 570LP | PE, DsRed, tdTomato, RFP, SYTOX Orange |
| 620/10 | 600LP | 7-AAD, m-Cherry, PE-Texas Red, PI, mRaspberry, Texas Red, PE/Dazzl 594, PE-CF594, PE-eFluor® 610, and ECD |
| 710/50 | 685LP | 7-AAD, Pe-Cy5.5, PI |
| 780/60 | 750LP | APC-Vio770, PE-Cy7, PE-Vio 770 |
| Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup. | ||
640nM Red laser
| Collection filter | Mirror | Fluorochromes |
|---|---|---|
| 670/14 | - | APC, Alexa Fluor 647, Cell Proliferation Dye eFluor 670, CellVue Maroon, CellVue Plum, Cy5, Cy5.5, DRAQ5, DRAQ7, Dylight 649, Dye Cycle Ruby, Fixable far red dead stain, Fixable Viability eFluor 660, Fx Cycle Far Red Stain, Oyster 650, SYTOX Red, TO-PRO-3, YO-PRO-3 |
| 730/45 | 710LP | Alexa Fluor 700, APC-Cy5.5, CellVue Burgundy, DRAQ7, Dye Cycle Ruby |
| 780/60 | 750LP | Alexa Fluor 750, APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, APC-H7, APC-Vio770, CellVue NIR780, DRAQ7, Fixable near-IR dead stain,, Fixable Viability eFluor 780 |
| Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup. | ||