Fortessa A

FortessaA filter set

355nM UV

Collection filterMirrorFluorochromes
450/50 - Calcein Blue AM, DAPI, Fixable Blue Dead Cell Stain, Indo-1 hi, Indo-1 AM, Hoechst 33258, NucBlue Live Cell Stain
530/30 505LP Alexa Fluor 430, Indo-1 lo, Qdot525 
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
Summary of the table's contents

405nM Violet

Collection filterMirrorFluorochromes
450/50 - Alexa Fluor 405, BD Horizon V450, BV 421, Calcein Violet, Calcein Violet 450AM, Celltrace Violet, CFP, DAPI, DyLight 405, DyeCycle Violet, eFluor 450, Fixable Violet Dead Cell Stain, Fixable Viability eFluor 450, FxCycle Violet, Hoechst (blue), Pacific Blue, PO-PRO-1, SYTOX Blue, VioBlue
525/50 475LP AmCyan, BD Horizon V500, Brilliant Violet 510, Fixable Aqua Dead cell stain, Fixable Viability eFluor 506, Krome Orange, Pacific Orange, Qdot 525, VioGreen
560/20* 550LP Qdot 565, Pacific Orange, Krome Orange 
585/15* 575LP Brilliant Violet 570, Fixable Yellow Dead Cell Stain, Qdot 585, Qdot 605
605/12* 595LP Brilliant Violet 605, eFluor605NC, Fixable Yellow Dead Cell Stain, Pacific Orange, Qdot 605
655/8* 635LP Brilliant Violet 650, Qdot 655
* These filters can be interchangeable- On the Violet laser BV 711 and Qdot 705 can be used with a 710/50 filter and a 685nm LP dichroic mirror. BV 785 and Qdot 800 can be used with a 780/60 filter and a 750nm LP dichroic mirror.
 
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
Summary of the table's contents

488nM Blue

Collection filterMirrorFluorochromes
530/30 505LP AlexaFluor 488, Calcein, CellVue Jade, CFSE, Cy2, DyLight 488, DyeCycle Green, eGFP,  eYFP , FITC, Fixable green dead cell stain, Fluo-3/Fluo-4, mCitrine, Oyster 488, Rhodamine 110,  Rhodamine 123,  SYTOX Green, TO-PRO-1, YO-PRO-1
675/20 660LP 7-AAD, PerCP, PI, (PE-Cy5**)
710/50 685LP 7-AAD, DRAQ5, Dye Cycle Ruby, PerCP-Cy5.5, PerCP-eFluor710, PI 

**PE-Cy5 is not recommended for use with APC.

Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
Summary of the table's contents

561nM Yellow Green

Collection filterMirrorFluorochromes
582/15 570LP PE, DsRed, tdTomato, RFP, SYTOX Orange
620/10 600LP 7-AAD, m-Cherry, PE-Texas Red, PI, mRaspberry, Texas Red
710/50 685LP 7-AAD, Pe-Cy5.5,  PI
780/60 750LP APC-Vio770, PE-Cy7, PE-Vio 770
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
Summary of the table's contents

633nM Red

Collection filterMirrorFluorochromes
670/14 - APC, Alexa Fluor 647, Cell Proliferation Dye eFluor 670, CellVue Maroon, CellVue Plum, Cy5, Cy5.5, DRAQ5, DRAQ7, Dylight 649, Dye Cycle Ruby, Fixable far red dead stain, Fixable Viability eFluor 660, Fx Cycle Far Red Stain, Oyster 650, SYTOX Red, TO-PRO-3, YO-PRO-3
720/40 710LP Alexa Fluor 700, APC-Cy5.5, CellVue Burgundy, DRAQ7, Dye Cycle Ruby
780/60 750LP Alexa Fluor 750, APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, APC-H7, APC-Vio770, CellVue NIR780, DRAQ7, Fixable near-IR dead stain,, Fixable Viability eFluor 780
Note: Not every fluorochrome will be able to be used in combination with the ones above, it is the responsibility of the user to research this during panel setup.
Summary of the table's contents