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My objectives
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Target audience
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The recommended course
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- To establish a LC-MS-based metabolic phenotyping laboratory
- To be able to competently operate LC-MS to acquire metabolic profiles of biological samples through hands-on experience
- To gain experience of untargeted and targeted LC-MS methodology, and development of these
- To design and conduct a metabolic profiling project using LC-MS
- Basic processing of LC-MS data, with some univariate and multivariate statistics
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- Early career researchers e.g. PhD students or postdoctoral researchers looking to move into metabonomics / metabolomics with LC-MS
- People who need an understanding of metabolic profiling with LC-MS
- More experienced LC-MS analysts who wish to troubleshoot their experiments
- Theoretical knowledge of LC-MS (don’t need to have operated one)
- Suitable for clinicians and researchers from industry who plan to use LC-MS for metabolic profiling
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- To establish an NMR spectroscopy-based metabolic phenotyping lab
- To be competently operate NMR spectrometer for high-throughput metabolic profiling
- To learn biomarker identification strategy (e.g. 2 dimensional NMR)
- To design and conduct a metabolic profiling project
- To be able to process the spectral data and perform multivariate statistical analysis
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- Early career researchers e.g. PhD students or postdoctoral researchers looking to move into metabonomics / metabolomics with NMR
- People who need an understanding of metabolic profiling with NMR
- More experienced NMR analysts who wish to troubleshoot their experiments
- Suitable for clinicians and researchers from industry who plan to use NMR for metabolic profiling
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- To understand the aims of untargeted metabolic profiling
- To understand the characteristics of metabolic profile data from NMR and LC-MS
- To know the basic steps involved in pre-processing NMR and LC-MS profiles
- To gain a good conceptual understanding of multivariate statistical methods like PCA and PLS
- To be able to apply processing and modelling techniques using common software packages
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- Analytical scientists with minimal statistics knowledge and no coding experience
- Bioinformaticians or statisticians wishing to understand more about techniques used in metabolic profiling
- Clinicians with minimal statistics knowledge and no coding experience
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