A Leica SP5 inverted confocal microscope with a tunable infrared laser for multiphoton imaging, Flim detector, motorised stage and full incubation chamber.

Advantages of this microscope

• Live multiphoton imaging for deep tissue penetration, maximum confocality
• 4 other lasers (Diode 405nm, Argon 488, Diode 543, Diode 633)
• Slide, dish and multiwell formats
• Flim detector for fluorescence lifetime imaging (Flim/FRET, intracellular environment)
• FRAP
• Automatic multipoint 4D imaging and stitching

Location

Sir Alexander Fleming building, SAF 408
SN: 5100000293

Quickstart guide

Quickstart guide - Confocal 4 Leica SP5 Inverted (PDF)‌‌

Quickstart guide - Multiphoton Laser (PDF)‌

Hardware configuration

Please see the FILM wiki page - CF4 hardware configuration

Leica Objectives

Offline software

• Leica LAS X Lite
• FIJI
• Becker and Hickl Leica MP-FLIM and D-FLIM handbook