@article{Blake:2026,
author = {Blake, I and Islam, MO and Fuller, B and Elwood, S and Pjolwat, S and Liu, J and Mira, Y and Faruque, ASG and Qadri, F and Haque, R and Taniuchi, M},
journal = {The Lancet Microbe},
title = {Integration of poliovirus and enteropathogen sewage surveillance in Dhaka Bangladesh: a longitudinal surveillance study June 2019 – June 2020},
year = {2026}
}

Download

TY  - JOUR
AB  - Environmental surveillance (ES) for poliovirus is a surveillance method used by the Global Polio Eradication Initiative (GPEI). ES will continue following certification of poliovirus eradication, potentially through its integration into other infectious disease surveillance programs. We evaluated TaqMan array cards (TAC) to detect poliovirus in sewage, whilst simultaneously testing for 11 other enteric pathogens and 34 markers of antimicrobial resistance (AMR) across 12 sites in Dhaka, Bangladesh. Sites were selected following mapping of the informal sewage network and a demographic survey of the population. Samples were collected before and after a bivalent oral polio vaccine (bOPV) campaign. 372 samples were collected over 379 days. A water-quality probe measured physicochemical properties of the sewage. A Multivariable mixed-effects Gamma Hurdle regression model was used to measure the association between enterovirus detection and concentration with site properties.   The highest concentration of Sabin-1 and -3 poliovirus was detected two weeks after the bOPV campaign (mean (SD) Sabin- 1 and -3 viral copies per L of sewage: 0·83 (2·13) and 0·84 (1·96)) , (vs baseline 0·05 (0·21) and 0·11 (0·50) respectively) [p=0·004: SL1, p=0·005: SL3] . Detection of enteroviruses was more likely with increasing levels of Total Dissolved Solids (mg/L) (aOR per absolute increase of 100 units 1·39 95%CI: 1·17 – 1·61). The median ES viral load of rotavirus was 0·567 (IQR 0·202-0·839), and this pathogen had the strongest correlation with respective concurrent clinical case incidence (cor = 0·828, p = 0·0017).  Thirty-one AMR genes of clinical significance were detected.When GPEI dissolves, poliovirus surveillance needs to be integrated into other surveillance programs. TAC may provide a method to screen suitable pathogens to survey in sewage alongside p
AU  - Blake,I
AU  - Islam,MO
AU  - Fuller,B
AU  - Elwood,S
AU  - Pjolwat,S
AU  - Liu,J
AU  - Mira,Y
AU  - Faruque,ASG
AU  - Qadri,F
AU  - Haque,R
AU  - Taniuchi,M
PY  - 2026///
SN  - 2666-5247
TI  - Integration of poliovirus and enteropathogen sewage surveillance in Dhaka Bangladesh: a longitudinal surveillance study June 2019 – June 2020
T2  - The Lancet Microbe
ER  - 

Download