@article{Wang:2026:10.1080/13510002.2026.2613534,
author = {Wang, M and He, Y and Hu, H and Wu, D and Liao, X and Gao, J and Gao, S and Yin, H and Chung, KF and Li, Q and Wang, K and Gao, W},
doi = {10.1080/13510002.2026.2613534},
journal = {Redox Rep},
title = {Protective role of fatty acid oxidation against epithelial barrier dysfunction in allergic asthma.},
url = {http://dx.doi.org/10.1080/13510002.2026.2613534},
volume = {31},
year = {2026}
}
TY - JOUR
AB - BACKGROUND: Fatty acid oxidation (FAO) is implicated in lung diseases, but its role in bronchial asthma is not fully understood. We investigated its effect on airway epithelial barrier integrity. METHODS: Using a house dust mite (HDM)-induced murine asthma model and HDM, IL-4, IL-13, or TNF-α stimulated human primary bronchial epithelial cells (BECs) and bronchial epithelial (Beas-2b) cells, we modulated FAO with L-carnitine (agonist) and Etomoxir (inhibitor). BECs and Beas-2b cells were infected with lentivirus-mediated CPT1A shRNA prior to stimulation. Barrier function, mitochondrial oxidative stress, inflammation, and metabolism were assessed. RESULTS: FAO level in lungs negatively correlated with increased inflammation and tissue injury in HDM-induced asthmatic mice (all p < 0.05), while positively regulating tight junction protein expression. In BECs and Beas-2b cells, Etomoxir treatment and CPT1A knockdown exacerbated the impairment of FAO caused by various stimulants (all p < 0.05). Furthermore, FAO negatively regulated HDM/cytokine-induced epithelial barrier damage, hyperactive inflammatory response, and mitochondrial dysfunction in Beas-2b cells (all p < 0.05). In contrast, treatment with L-carnitine significantly alleviated these pathophysiological features in both in vivo and in vitro models. CONCLUSION: FAO plays a protective role in the occurrence and development of asthma by maintaining airway epithelial cell homeostasis and barrier function.
AU - Wang,M
AU - He,Y
AU - Hu,H
AU - Wu,D
AU - Liao,X
AU - Gao,J
AU - Gao,S
AU - Yin,H
AU - Chung,KF
AU - Li,Q
AU - Wang,K
AU - Gao,W
DO - 10.1080/13510002.2026.2613534
PY - 2026///
TI - Protective role of fatty acid oxidation against epithelial barrier dysfunction in allergic asthma.
T2 - Redox Rep
UR - http://dx.doi.org/10.1080/13510002.2026.2613534
UR - https://www.ncbi.nlm.nih.gov/pubmed/41555513
VL - 31
ER -