Crystallisation Screens
We prepare 14 commercial screens in 96-well plates, ready for use with the Mosquito crystallization robot. In addition, we also create optimization screens based on the four-corners strategy and provide Hampton's additive screen in 110 µl aliquots.
JBS Cryo (ICL11) has been discontinued by the manufacturer. We have replaced it with a similar screen, Wizard Cryo by Rigaku Reagents (ICL15). If you really, really need JBS Cryo (or any other custom screen), Molecular Dimensions will prepare any 96x 10 ml custom kit for £400 + £15 delivery. Lead time is ~5 working days from receipt of PO. Contact Jeanette Hobbs if interested.
Commercial Crystallisation Screens
| Plate ID | Name + Composition | Company |
|---|---|---|
| ICL0* | Structure Screens 1 and 2 | Molecular Dimensions |
| ICL2 | Wizard 1 and 2 | Rigaku Reagents/Molecular Dimensions |
| ICL3N** | PEG/Ion 1 and 2 | Hampton Research |
| ICL4 | Index | Hampton Research |
| ICL5 | SaltRx 1 and 2 | Hampton Research |
| ICL6 | MemStart + MemSys | Molecular Dimensions |
| ICL7 | PACT premier | Molecular Dimensions |
| ICL8 | JCSG+ | Molecular Dimensions |
| ICL9 | MemGold | Molecular Dimensions |
| ICL10N** | Wizard 3 and 4 | Rigaku Reagents/Molecular Dimensions |
| ICL12 | Proplex | Molecular Dimensions |
| ICL13 | Morpheus | Molecular Dimensions |
| ICL14 | PGA screen | Molecular Dimensions |
| ICL15 | Wizard Cryo | Rigaku Reagents/Molecular Dimensions |
| ICL16** | Natrix 1 and 2 | Hampton Research |
| ICL17 | BCS screen | Molecular Dimensions |
| ICL18 | MemGold2 | Molecular Dimensions |
| ICL19 | MemMeso | Molecular Dimensions |
| ICL20 | MemTrans | Molecular Dimensions |
| ICL21 | MemChannel | Molecular Dimensions |
| Detergent Screen | Detergent Screen | Hampton Research |
A list of all screen compositions can be found in the binder next to the Mosquito. MIDAS is available from 13 Nov 2014 while supplies last. Order an empty MRC plate on Sharepoint and notify the facility manager that you'd like to have a MIDAS screen.
* December 2015: ICL0 from Molecular Dimensions has replaced ICL1 (Crystal Screens 1 & 2 from Hampton Research). These screens are identical with the exception of 5 conditions (highlighted in the binder)
** April 2016: ICL3N, ICL10N and ICL16 replace old ICL3 and ICL10 screens.
Overlap among screens
There's a high degree of redundancy among the screens available in the facility. Some screens were designed based on the same principles and crystallization data, while others are explicitly based on earlier screens.
- ICL7 – PACT builds upon PEG/ion (ICL3a, ICL10b), comprising a 24-solution PEG/pH screen covering the range 4 to 9, a 24-solution cation/PEG screen and a 48-solution anion/PEG screen.
- ICL8 – The JCSG+ screen takes the 66 best solutions identified by the Joint Center for Structural Genomics and adds 30 conditions from Index (ICL4) to maximize chemical and pH diversity.
Charging structure
We operate on a cost recovery basis (charges for Imperial users). The prices charged for the plates include the use of the Mosquito. Detergent, Additive screens and preparation of a four-corners optimization screens cost extra. We are happy to provide plates to external users. Please contact the facility manager for details.