@article{Rakotomalala:2025:10.1038/s41467-025-64027-4,
author = {Rakotomalala, Robinson D and Pennisi, I and Cavuto, M and Kiemde, F and Chamai, M and Yrgnur, Some D and Quigley, E and Malpartida, Cardenas K and Ousmane, Ndiath M and Correa, S and Darboe, B and Stewart, L and Georgiou, P and Baldeh, M and Tinto, H and Cunnington, A and Erhart, A and DAlessandro, U and Rodriguez, Manzano J and On, behalf of the NIHR Global Health Research Group on Digital Diagnostics for African Health Systems},
doi = {10.1038/s41467-025-64027-4},
journal = {Nature Communications},
title = {Sensitive near point-of-care detection ofasymptomatic and sub-microscopicPlasmodium falciparum infections in Africanendemic countries},
url = {http://dx.doi.org/10.1038/s41467-025-64027-4},
year = {2025}
}

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TY  - JOUR
AB  - Limited diagnostic capacity for detecting asymptomatic malaria infections with low parasite densities hinders elimination efforts in Africa. Here, we adapt a near point-of-care, LAMP-based diagnostic platform for malaria diagnosis using capillary blood. This Pan/Pf detection method meets the Malaria Eradication Research Agenda (malERA) criteria for community-level screening, witha limit of detection of 0.6 parasites/μL and a sample-to-result time under 45 minutes. We evaluate its performance on 672 capillary blood samples collected at the community level in The Gambia and Burkina Faso, including 146Plasmodium falciparum positives confirmed by qPCR. The diagnostic platform achieves 95.2% sensitivity (95% CI: 90.4–98.1) and 96.8% specificity (95% CI: 94.9–98.0). It also detects 94.9% (130/137) of asymptomatic infections and 95.3% (41/43) of sub-microscopic cases (<16 parasites/μL), outperforming expert microscopy (70.1% and 0%) and rapid diagnostic tests (49.6% and 4.7%).  This field-deployable molecular diagnostic method offers a sensitive, scalable solution to support test-and-treat strategies for malaria elimination across Africa.
AU  - Rakotomalala,Robinson D
AU  - Pennisi,I
AU  - Cavuto,M
AU  - Kiemde,F
AU  - Chamai,M
AU  - Yrgnur,Some D
AU  - Quigley,E
AU  - Malpartida,Cardenas K
AU  - Ousmane,Ndiath M
AU  - Correa,S
AU  - Darboe,B
AU  - Stewart,L
AU  - Georgiou,P
AU  - Baldeh,M
AU  - Tinto,H
AU  - Cunnington,A
AU  - Erhart,A
AU  - DAlessandro,U
AU  - Rodriguez,Manzano J
AU  - On,behalf of the NIHR Global Health Research Group on Digital Diagnostics for African Health Systems
DO  - 10.1038/s41467-025-64027-4
PY  - 2025///
SN  - 2041-1723
TI  - Sensitive near point-of-care detection ofasymptomatic and sub-microscopicPlasmodium falciparum infections in Africanendemic countries
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-025-64027-4
ER  - 

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