@article{Chen:2026:10.1073/pnas.2523470123,
author = {Chen, Y-F and Lin, K-Y and Huang, C-Y and Hou, L-Y and Yuen, ELH and Sun, W-CJ and Chiang, B-J and Chang, C-W and Wang, H-Y and Bozkurt, TO and Wu, C-H},
doi = {10.1073/pnas.2523470123},
journal = {Proc Natl Acad Sci U S A},
title = {Subcellular calcium dynamics and organelle perturbations in resistosome-mediated cell death.},
url = {http://dx.doi.org/10.1073/pnas.2523470123},
volume = {123},
year = {2026}
}

Download

TY  - JOUR
AB  - Plant nucleotide-binding domain leucine-rich repeat-containing (NLR) proteins act as intracellular immune receptors that assemble into resistosomes to execute immune responses. However, the subcellular processes during cell death following resistosome activation remain unclear. Here, we visualized the changes in calcium signaling and organelle behavior after activation of the NRC4 (NLR required for cell death 4) resistosome. We found that NRC4 membrane enrichment coincided with calcium influx. This is followed by sequential mitochondria and plastid disruption, endoplasmic reticulum fragmentation, and cytoskeleton depolymerization. Subsequent loss of plasma membrane integrity, nuclear shrinkage, and vacuolar collapse mark the terminal stage of cell death. Our findings reveal a spatiotemporally resolved cascade of subcellular events downstream of resistosome activation, providing mechanistic insight into the execution phase of plant immune cell death.
AU  - Chen,Y-F
AU  - Lin,K-Y
AU  - Huang,C-Y
AU  - Hou,L-Y
AU  - Yuen,ELH
AU  - Sun,W-CJ
AU  - Chiang,B-J
AU  - Chang,C-W
AU  - Wang,H-Y
AU  - Bozkurt,TO
AU  - Wu,C-H
DO  - 10.1073/pnas.2523470123
PY  - 2026///
TI  - Subcellular calcium dynamics and organelle perturbations in resistosome-mediated cell death.
T2  - Proc Natl Acad Sci U S A
UR  - http://dx.doi.org/10.1073/pnas.2523470123
UR  - https://www.ncbi.nlm.nih.gov/pubmed/41849389
VL  - 123
ER  - 

Download